A.1 Total DNA = 3.2 Kbp = 3200 bp
Adenine = 820
According to Chargaffs rule [ A ] =[T],[G]=[C]
So, Thymine =820
Therefore, total AT content =820+820=1640 GC content =3200−1640=1560
So, cytosine = 1560/2 = 780
A.2 The term cDNA refers to complementary DNA. cDNA is known to be synthesized, or manufactured from an mRNA or messenger RNA template. It is synthesized in a reaction that is catalyzed by the reverse transcriptase and DNA polymerase enzymes. Essential to note is that cDNA is usually used to clone eukaryotic genes in prokaryotes.